The effect of vitamin E on the lipid environment of rat hepatocyte membranes

Tocopherol is one of the known beneficial natural antioxidants ensuring optimal level functioning mammalian organisms. Numerous in vitro and vivo experiments have shown that biological role vitamin E to prevent development pathologies caused by oxidative stress. In particular, enzymatic factors lipid peroxidation related inflammation as a result eicosanoid synthesis was clearly shown. We studied changes structural functional state hepatocyte membranes classical model E-hypovitaminosis long-term (70 days) insufficient intake diet rats. The test components were determined spectrophotometrically after appropriate chromatographic procedures. amount total individual leukotrienes ELISA. Prolonged tocopherol deficiency rats 49.4% decrease tocopherol, more than 27.0% – cholesterol. Of 8 phospholipids studied, 6 showed significant changes: cardiolipin phosphatidylserine, an increase phosphatidylethanolamine 3.24 times, increse lysophosphatidylcholine 86.9%, phospha­tidylcholine 52.8%, sphingomyelin 30.6%, relative control. There levels unsaturated fatty acids playing disorders cells affecting metabolism ecosanoids derived from arachidonic acid 5-lipoxygenase oxidation pathway. Changes cysteinyl revealed. Restoration returns most indicators such cholesterol, polyunsaturated control activates processes sequential conversion body obtained results indicate potentiating effect on metabolic whole hepatocytes metabolism. degree allows influence course inflammatory associated with eicosanoids, not only through impact precursors, but also utilization metabolites, including leukotrienes.